Signalling
Part:BBa_K1180003:Design
Designed by: Chen Xi, Niu Yuchen,Dai Yimei Group: iGEM13_NJU_China (2013-09-24)
Fusion protein of lamp 2b and pre-s1 for liver targeting of the exosome
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1390
Illegal SpeI site found at 703
Illegal PstI site found at 1087
Illegal PstI site found at 1473 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 703
Illegal PstI site found at 1087
Illegal PstI site found at 1473 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1872
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1390
Illegal SpeI site found at 703
Illegal PstI site found at 1087
Illegal PstI site found at 1473 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1390
Illegal SpeI site found at 703
Illegal PstI site found at 1087
Illegal PstI site found at 1473 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 334
Illegal BsaI site found at 1567
Design Notes
We tried to make the pre-S1 sequence as short as possible in order to avoid any immune response which might be induced by the fusion protein.
Source
The sequence of our part come from the protein lamp 2b and pre-S1 from HBV envelope protein.